Comprehensive assessment of chemokine expression profiles by flow cytometry.

P-12: Direct Evaluation of HspA2 in Fertile and Infertile Individuals

Background: Heat-shock protein A2 (HspA2) is a testis-specific member of the HSP70 family known to play a critical role in spermatogenic cell differentiation. HSPA2 is correlated with sperm maturity, function and fertility, and diminished expression of HSPA2 results in abnormal sperm maturity and infertility. The aim of this study was to assess maturity of ejaculated spermatozoa via comparing p...

Comparative Analysis of Expression of Chemokoine Receptors CXCR4, CXCR6, CCR1 and CX3CR in Human Adipose-Drived Mesenchymal Stem Cell with Valproic Acid

Introduction: Chemokine receptors are found on the surface of stem cells. There have been 19 distinct chemokine receptors described in mammals. Chemokines are major players in migration and homing. Therefore, changes in their levels or function can help us to increase the migratory potential of these cells. Valproic acid differs in structure from other drugs in common use. The way in which Va...

Flow Cytometry: A Novel Approach for Indirect Assessment of Protamine Deficiency by CMA3 Staining, Taking into Account the Presence of M540 or Apoptotic Bodies

CCR3, CCR5, CCR8 and CXCR3 expression in memory T helper cells from allergic rhinitis patients, asymptomatically sensitized and healthy individuals

BACKGROUND Chemokine receptors have been suggested to be preferentially expressed on CD4+ T cells with CCR3 and CCR8 linked to the T helper (Th) 2 subset and CCR5 and CXCR3 to the Th1 subset, however this remains controversial. OBJECTIVE Our aim was to compare the CCR3, CCR5, CCR8 and CXCR3 expression in memory Th cells from allergic, asymptomatically sensitized and healthy individuals. MET...

P-6: Flow Cytometry: Assessment of Protamine Deficiency by CMA3 Staining and Apoptotic Bodies

Background: Chromomycin A3 (CMA3) staining, with the slide method or fluorescence microscopy, is widely used for indirect assessment of protamine deficiency in a semen sample. Flow cytometry is the most suitable tool which to improve assessment accuracy, both in terms of statistical analysis and to prevent observer variation. This study provides a simple procedure to account for merocyanine 540...